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Image Search Results
Journal: International Journal of Molecular Sciences
Article Title: Nitric Oxide Implication in Potato Immunity to Phytophthora infestans via Modifications of Histone H3/H4 Methylation Patterns on Defense Genes
doi: 10.3390/ijms23074051
Figure Lengend Snippet: NO-mediated changes in histone arginine methylation PRMT5 expression ( A ), PRMT5 activity ( B ) distribution levels of H4R3sme2 on the promoter of NPR1 , WRKY1 , PR1 , and R3a , respectively ( C ). RT-qPCR and ELISA tests were performed in potato leaves (at 1–48 h) after treatment with GSNO, GSH, cPTIO, water, or avr P. infestans inoculation, respectively. ChIP–qPCR analyses were performed in potato leaves at selected time points (3–24 h), after treatment with GSNO, GSH, cPTIO, water, or avr P. infestans inoculation, respectively. Data are presented as X-fold enrichment . The relative amounts of immunoprecipitated chromatin fragments (as determined by real-time PCR) from the above variants of treatment were compared with the reference (arbitrarily set to 1). Each experiment included at least three independent measurements per sample. P values for each sample combination were calculated using ANOVA and mean values were compared using the Tukey–Kramer test (α = 0.05 (*) and α = 0.01 (**)).
Article Snippet: The level of PRMT5 activity was determined using an
Techniques: Methylation, Expressing, Activity Assay, Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay, ChIP-qPCR, Immunoprecipitation, Real-time Polymerase Chain Reaction
Journal: International Journal of Molecular Sciences
Article Title: Nitric Oxide Implication in Potato Immunity to Phytophthora infestans via Modifications of Histone H3/H4 Methylation Patterns on Defense Genes
doi: 10.3390/ijms23074051
Figure Lengend Snippet: PRMT5 inhibitor (GSK3326595) drastically reduces PRMT5 activity ( A ) and causes a dose-dependent decrease in the total amount of H4R3sme2-marked histone proteins ( B ). ELISA of PRMT5 histone protein activity was performed using potato leaves after the following separate treatments: DMSO; inhibitor in DMSO; DMSO followed by avr P. infestans (6 hpi) or inhibitor in DMSO followed by avr P. infestans (6 hpi), respectively. For Western blot analysis, potato leaves were treated with increasing concentrations (50, 100, 200 µM) of GSK3326595 in DMSO or DMSO as the control. Total histone proteins were probed with H4R3sme2-specific antibodies and H4 histone from the calf thymus as a loading control. Values represent the means of data ± SD of at least three independent experiments. Asterisks indicate values that differ significantly from DMSO at α < 0.05 (*) and α < 0.01 (**).
Article Snippet: The level of PRMT5 activity was determined using an
Techniques: Activity Assay, Enzyme-linked Immunosorbent Assay, Western Blot, Control
Journal: International Journal of Molecular Sciences
Article Title: Nitric Oxide Implication in Potato Immunity to Phytophthora infestans via Modifications of Histone H3/H4 Methylation Patterns on Defense Genes
doi: 10.3390/ijms23074051
Figure Lengend Snippet: PRMT5 activity is required in potato resistance to avr P. infestans . Potato leaf surface (with or without PRMT5 inhibitor) covered by late blight symptoms ( A ), Pitef1 gene expression ( B ) and measurement of lesion size at 72 hpi after challenge inoculation with avirulent or virulent P. infestans (MP977) ( C ). Gene expression of R3a and HSR203J ( D ) and TUNEL assay at 6 hpi ( E ). Categorical scatter plots show lesion diameters of twelve inoculated sites from three biological replicates marked with three colors. Gene expression values represent the means of data ± SD of at least three independent experiments, each with at least three biological replicates. Asterisks indicate values that differ significantly from mock-inoculated (water treatment) potato leaves at α < 0.05 (*) and α < 0.01 (**).
Article Snippet: The level of PRMT5 activity was determined using an
Techniques: Activity Assay, Gene Expression, TUNEL Assay